Fig. (2) Time course of the phsophorylation of the peptide substrate by unphosphorylated Met. 4 nM of unphosphorylated c-Met was incubated with 1 µM of peptide substrate Biotin- EQEDEPEGDYFEWLE-amide and 200 µM ATP. The reaction was carried out at 25 °C in 50 mM Tris-HCl, 10 mM MgCl₂ ,100 mM NaCl, 0.1 mg/ml BSA, 5mM DTT, pH 7.8. Points are duplicates and the data were fit to a line.