Fig. (3) Comparison of dose-dependent inhibition of phosphorylated and unphosphorylated c-Met by small molecule inhibitors. 4 nM of the unphosphorylated was incubated with 1 µM of peptide substrate, 200 µM ATP and small molecule inhibitors (filled triangles) for 90 minutes at 25 °C. 0.25 nM of the phosphorylated c-Met was mixed with1 µM of peptide substrate 80 µM ATP and small molecule inhibitors (open squares) for 90 minutes at 25 °C. Hit 7 is from Table 2 and Hit 3 is from Table 3. Points are duplicates normalized to 100% for the uninhibited kinase. Data were fit to a hyperbola on logarithmic scale.