Fig. (2)
Enzyme kinetics. (
a
) At the optimal pH of 5.9, the enzyme had a K
m
of 144 µM and a V
max
of 5.74 pmol/min. (
b
) At an acidic lysosomal pH of 4.5, the enzyme had a K
m
of 102 µM and a V
max
of 2.76 pmol/min.
