|
Ramm (Ref. #24) |
Price (Ref. #25) |
Mitchell (Ref. #4) |
Radio (Ref. #5) |
Yeyeodu (This paper) |
Instrument |
IN Cell Analyser 1000 (Amersham) |
Automated microscope stage (Prior Scientific) |
Ludl stage |
ArrayScan V (Cellomics) |
BD Pathway 855 |
Acquisition software |
(included) |
(Media Cybernetics) |
VolumeScan (Vaytek) |
(included) |
BD AttoVision 1.6 |
Autofocus |
yes |
yes |
yes |
yes |
yes |
Image intensity resolution |
12 bit |
NR |
24 bit |
NR |
12 bit |
Analysis software |
(included) |
custom algorithm (Advansoft) in ImagePre Plus 4.1 (Media Cybernetics) |
custom algorithm (detailed in text) in ImagePro Plus |
(included) |
BD AttoVision 1.6 with Neurite Outgrowth Module |
Neurite defined |
~2x cell diam. (30m) |
NR |
NR |
~2x total neurite length in cells w/o NGF (> 20 µm) |
>1.5x cell width |
Parameters measured |
|
|
|
|
|
Total neurite length |
yes |
|
yes |
|
yes |
Total cell body area |
yes |
|
|
|
|
Num. neurites |
yes |
|
|
|
yes |
Avg cell diameter |
yes |
|
|
|
|
Num. nuclei or cells |
|
yes |
yes |
yes |
yes |
Individual neurite length |
|
yes |
|
|
|
Num. branches |
|
yes |
|
yes |
yes |
Maximum neurite length |
|
|
yes |
|
yes |
Neurites/cell |
|
|
|
|
yes |
Cell body area |
|
|
|
yes |
|
Parameters derived |
|
|
|
|
|
% neurite bearing cells |
|
yes |
yes |
|
|
Num. cells |
yes |
|
|
|
yes |
Mean neurite length |
yes |
yes |
yes |
|
yes |
Total neurite length/cell |
yes |
yes |
|
|
yes |
Mean neurite length/cell |
|
|
|
yes |
yes |