A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter

Fig. (2) NHR LinkLight Assay. a, A schematic drawing of NHR-TEV and NCOA1-pLuc interaction LinkLight assay. b, U-2 OS cells stably expressing NCOA1-pLuc and transiently expressing RXRα-TEV genes showed dose-response curve in response to the ligand retinoic acid. c, U-2 OS cells stably expressing NCOA1-pLuc and transiently expressing ERα-TEV genes showed dose-response curve in response to the ligand β-estradiol. d, U-2 OS cells stably expressing NCOA1-pLuc and transiently expressing PXR-TEV genes showed dose-response curves in response to the ligand rifampicin. Data points are represented as mean ± SD from n=3. EC₅₀ values were derived from non-linear regression with the best-fit equation.