Table 1: Comparison of EC50 Values of Four Different Assay Formats. The ADRB2-TEV Construct was Used in LinkLight, cAMP, FLIPR, and CRE-Luc Assays. GraphPad Prism 5 was Used to Plot all the Data and EC50 Values were Derived from Non-Linear Regression with the Best-Fit Equation
|
LinkLight Assay |
cAMP Assay |
FLIPR Assay |
CRE-Luc Assay |
| EC50 (Isoproterenol) |
3.72e-009 |
3.07e-009 |
1.65e-009 |
2.0e-009 |
| Hill Slope |
1.260 |
1.552 |
0.4067 |
0.9552 |