Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells

Table 2: Comparison of Potency (µM, IC₅₀) of Commercial Ordered Compounds in ELISA-Based TNF-α, HTRF-Based TNF-α, and Cell Viability Assays

Compound Name	ELISA	HTRF	Cytotoxicity
Bay 11-7085	0.33 ± 0.15	1.45 ± 0.11	7.72
BTO-1	1.95 ± 0.7	4.61 ± 0.37	12.9
CCG-2046	0.64 ± 0.09	2.44 ± 0.002	8.66
Dobutamine hydrochloride	0.09 ± 0.05	0.21 ± 0.02	Inactive
Ellipticine	0.44 ± 0.07	1.38 ± 0.22	10.9
Histamine dihydrochloride	0.71 ± 0.31	1.37 ± 0.003	Inactive
IRAK-1/4 Inhibitor I	0.54 ± 0.1	3.14 ± 1.69	Inactive
PD 169316	0.01 ± 0.001	0.07 ± 0.01	25.9

Each value in ELISA and HTRF-based assays is the mean ± SD of the results from two experiments in duplicates. Cell viability assay was performed in duplicates from one experiment.