Fig. (1) Characterization of GST-GAPDHS. (A) SDS-PAGE analysis of purified recombinant human GST–GAPDHS. GST-GAPDHS was expressed in the gapA-deficient E. coli strain DS112 and purified using glutathione-Sepharose. Coomassie-Blue stained 4-12% Bis-Tris gel, lane M, Prestained protein markers (Novex); lane 1, recombinant human GST-GAPDHS (2 µg). (B) Size exclusion chromatography of purified GST-GAPDHS. Purified human GST-GAPDHS was loaded on a Superose 12 column at 0.5 ml/min in 50 mM potassium phosphate pH 7.0, 400 mM NaCl buffer. The molecular size of the peaks indicated by the numbers were estimated as described previously [22].