Table 1: Automation Protocol for GAPDHS HTS Assay

Step	Event	Parameter	Description
1	Pre-spot	0.5 µL	Add DMSO/test compound to 384-well plates using Biomek NX
2	Dispense	25 µL	Add GAPDHS and NAD solution to a set of ten plates using Multidrop 384
3	Incubate	15 min	Room temperature
4	Dispense	25 µL	Add GAP substrate to one plate using Multidrop 384
5	Read	∆A340	SpectraMax plate reader; 5 min kinetic read
6	Dispense	25 µL	Repeat steps 4 and 5 for remaining nine plates