| Generation | Template RNA (pmol) | hMG (pmol) | Reaction time (min) | PCR (cycle) | Recovered RNA (pmol) |
|---|---|---|---|---|---|
| 1 | 2000 | 500 | 60 | 15 | 5688 |
| 2 | 2844 | 711 | 45 | 15 | 6004 |
| 3 | 2002 | 501 | 30 | 15 | 5072 |
| 4 | 2454 | 614 | 20 | 15 | 5508 |
| 5 | 2754 | 689 | 10 | 15 | 5238 |
| 6 | 2619 | 655 | 5 | 15 | 5358 |
| 7 | 2679 | 670 | 2.5 | 15 | 5400 |
| 8 | 2524 | 631 | 1.5 | 15 | 4692 |
| 9 | 2346 | 587 | 1 | 15 | 4836 |
| 10 | 2418 | 605 | 0.5 | 15 | 5040 |
| 11 | 1000 | 250 | 0.5 | 10 | 1212 |
| 12 | 500 | 125 | 0.5 | 10 | 645 |