Fig. (5). RNA analysis of neutrophils from a patient with CGD, his mother, and a control subject Single-stranded cDNA was synthesized from neutrophil RNA obtained from the patient, his mother, and a control subject. cDNA was PCR-amplified using a sense primer specific for exon 4 and an antisense primer specific for exon 8 of the CYBB gene. The products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size markers are shown on the left and the numbers on the right indicate differ-ently spliced products. Although some normally splice transcripts were identified, most PCR products amplified from the patient lacked exon 5 or exons 5 and 6. Normally spliced transcripts and transcripts lacking exon 5 were identified in the mother.