Fig. (11) Structural stability of HT7 and HT2. A. Temperature
dependence of CD signal at 224 nm. Denaturation temperatures
(Tm) were determined by fitting the data to a simple two-state transition
model. B. Effect of urea on ligand binding activity. Proteins
were exposed to urea for 16 h at 25 °C and the amount of properly
folded protein remaining (relative to no treatment) was calculated
based on sensitivity to thermolysin-induced proteolysis .