Fig. (13) Pulse labeling of different ribosomal populations in U2OS cells. Cells stably expressing RPS9-HT7 were serum starved for 18 h, and then labeled using the TMR-ligand (panels A, D). After recovery for 3 h (panel B) or 24 h (panel E) in complete media, newly synthesized populations of RSP-HT7 were pulse labeled using the Oregon Green-ligand. Panel C is an overlay of panels A and B. Panel F is an overlay of panels D and E. Scale bar = 20 ┬Ám.