Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands

Fig. (5) TMR-ligand binding kinetics for HT2. Reactions between HT2, Y273L, K175M/C176G, or H272F (40 nM) and the TMR-ligand (2.5 nM) were carried out at 25 °C and monitored for binding over time using FP.