Table 1: Comparison of Success Rates of Soluble Expression of Recombinant Proteins which Derived from Various Expression Vectors

Fusion tag Expression Solubility HaloTag Vector Expression Solubility
pHis: His-tag1 59.5% 43.2% pFN18A3 (23) 73.9% 56.5%
pHis: His-tag2 54.0% 39.6% pGW-nHalo3 (74) 82.4% 70.3%
pMBP: ΔSP-MBP2 72.7% 70.1% pFN19A3 (52) 75.0% 69.2%
pSP-MBP: MBP2 64.7% 43.9% pFC203 (67) 67.2% 61.2%
pDsbA: DsbA2 58.8% 47.6% pFC14A4 (10) 80.0% N/A
pEXP7: GST2 49.7% 42.8% HaloTag (average) 75.2% 65.7%

1 Genome-wide protein expression and purification of S. pneumoniae proteome success rates were calculated based on efforts applied to 1529 destination clones [14].
2 Putative proteases derived from S. pneumoniae TIGR4, B. anthracis Ames and Y. pestis KIM. Success rates were calculated based on 187 destina-tion clones [18].
3 Combination of protein sets (23-80 clones) of DNA binding proteins, Type 3 secretion system, Type 6 secretion system and/or randomly selected proteins in E. coli, S, pneumoniae TIGR4, Y. pestis B. anthracis, Ames, and Burkholderia mallei ATCC23344.
4 10 H1N1 proteins were used in the study and solubility information is not available. The numbers in parentheses are the number of clones in the study.