Table 1: Assay Protocol

Step	Parameter	Value	Description
1	Reagent	2 ml	1 nM glCK in assay buffer
2	Reagent	23 nl	Pin transfer compounds in DMSO
3	Time	15 min	Room temperature incubation
4a	Reagent	1 ml	600 mM CP, 600 mM ADP in assay buffer
4b	Reagent	1 ml	ATPLiteTM detection reagent
5	Time	20 min	Room temperature incubation
6	Detection		ViewLux plate reader luminescence mode