Fig. (2) Comparison of concentration responses of 2-iodomelatonin (a MT1 melatonin receptor agonist) determined in the single assay to these in the multiplexed assay. (A) EC50 values of 2-iodomelatonin were 19.4 nM and 13.8 nM measured in the FLIPR calcium assay in the single assay format (FLIPR) and multiplexed assay format (Multip), respectively. (B) EC50 values of 2-iodomelatonin were 5.38 pM and 3.48 pM determined in the NFAT beta-lactamase reporter gene assay in the single assay format (BLA) and multiplexed assay format (Multip),respectively.