Fig. (6) Concentration responses of five representative active compounds identified from the compound library screen with this multiplexed assay.(A) The EC50 values of Compound # 249 in the NFAT beta-lactamase assay were 0.08 and 0.56 nM in the single assay format and multiplexed assay format, respectively. (B) The EC50 values of Compound #250 in the NFAT beta-lactamase assay were 1.08 and 0.42 nM the single assay format and multiplexed assay format, respectively. (C) The EC50 values of Compound # 251 in the NFAT beta-lactamase assay were 2.46 and 3.69 nM in the single assay format and multiplexed assay format,respectively.(D) The EC50 values of Compound #252 in the NFAT betalactamase assay were 3.00 and 8.84 nM in the single assay format and multiplexed assay format, respectively. (E) The EC50 values of Compound #253 in the NFAT beta-lactamase assay were 2.25 and 2.76 nM in the single assay format and multiplexed assay format, respectively.