Fig. (3A) RWG-based competition assay: Inhibition of His-
Nef/GST-SH3 complex formation by DLC27. The binding of 150
nM His-Nef to 5 µM GST-SH3 immobilized to the biosensor surface
was monitored by RWG in the presence of 1 % (v/v) DMSO or
50 µM DLC27 in PBS in real-time. (B) RWG-based direct binding
assay: Binding kinetics of D14 and DLC27 to His-Nef. The peak
wavelength shift (ΔPWV) was assessed after the addition of 50µM
DLC27 or D14 in 1 % (v/v) DMSO in PBS to 5 µM immobilized
His-Nef in real-time. (C) Binding kinetics of D14 and DLC27 to
GST-SH3. The !PWV was assessed after the addition of 50 µM
DLC27 or D14 in 1% (v/v) DMSO in PBS to 5 µM immobilized
GST-SH3 in real-time.