Fig. (3A) RWG-based competition assay: Inhibition of His- Nef/GST-SH3 complex formation by DLC27. The binding of 150 nM His-Nef to 5 µM GST-SH3 immobilized to the biosensor surface was monitored by RWG in the presence of 1 % (v/v) DMSO or 50 µM DLC27 in PBS in real-time. (B) RWG-based direct binding assay: Binding kinetics of D14 and DLC27 to His-Nef. The peak wavelength shift (ΔPWV) was assessed after the addition of 50µM DLC27 or D14 in 1 % (v/v) DMSO in PBS to 5 µM immobilized His-Nef in real-time. (C) Binding kinetics of D14 and DLC27 to GST-SH3. The !PWV was assessed after the addition of 50 µM DLC27 or D14 in 1% (v/v) DMSO in PBS to 5 µM immobilized GST-SH3 in real-time.