| Name | Assay Principle | Detection Technology | Provider |
|---|---|---|---|
| In-cell Western | Using ERK1/2 primary antibodies and infrared-labeled secondary antibodies sandwich assay to detect infrared signal in fixed cells | Infrared fluorescence | LI-COR Biosciences |
| MSD ERK Activation Assay | Using ERK1/2 antibody conjugated with an electrochemiluminescent compound and capture antibody for phosphorylated ERK1/2 coated in microplate wells for detection | Electrochemiluminesence | Meso-Scale Discovery |
| AlphaScreen SureFire™Cellular ERK Assay | Using antibody-coated AlphaScreen beads to generate a fluorescent signal when in close proximity due to binding of phospho-ERK1/2 (Thr202/Tyr204) | Fluorescence emission | TGR BioSciences & Perkin Elmer |
| Cayman ERK/MAPK (Thr202/Tyr204) Cell-based Assay | A sandwich assay using anti-Thr202/Tyr204 primary antibody with a DyLight conjugated secondary antibody | Immunofluorescence staining | Cayman |
| Phospho-ERK (Cellul'erk) Assay | A sandwich assay format using 2 different specific monoclonal antibodies: the anti-phospho-ERK antibody labeled with d2 and the anti-ERK antibody labeled with Eu3+-cryptate. | HTRF | Cisbio |
| LanthaScreen® Activity Assay | Terbium (Tb)-labeled antibody binds the dually phosphorylated (Thr185, Tyr187) ERK2-GFP fusion protein expressed in cells. This association allows energy transfer to occur between the excited state Tb fluorophore and GFP, leading to an increase in TR-FRET signal | TR-FRET | Life Technologies |