Name |
Assay
Principle |
Detection
Technology |
Provider |
In-cell
Western |
Using
ERK1/2 primary antibodies and infrared-labeled secondary antibodies
sandwich assay to detect infrared signal in fixed cells |
Infrared
fluorescence |
LI-COR
Biosciences |
MSD ERK
Activation Assay |
Using
ERK1/2 antibody conjugated with an electrochemiluminescent compound
and capture antibody for phosphorylated ERK1/2 coated in microplate
wells for detection |
Electrochemiluminesence |
Meso-Scale
Discovery |
AlphaScreen
SureFire™Cellular ERK Assay |
Using
antibody-coated AlphaScreen beads to generate a fluorescent signal
when in close proximity due to binding of phospho-ERK1/2
(Thr202/Tyr204) |
Fluorescence emission |
TGR
BioSciences & Perkin Elmer |
Cayman ERK/MAPK
(Thr202/Tyr204) Cell-based Assay |
A sandwich
assay using anti-Thr202/Tyr204 primary antibody with a DyLight
conjugated secondary antibody |
Immunofluorescence staining |
Cayman |
Phospho-ERK
(Cellul'erk) Assay |
A sandwich
assay format using 2 different specific monoclonal antibodies: the
anti-phospho-ERK antibody labeled with d2 and the anti-ERK antibody
labeled with Eu3+-cryptate. |
HTRF |
Cisbio |
LanthaScreen® Activity Assay |
Terbium
(Tb)-labeled antibody binds the dually phosphorylated (Thr185,
Tyr187) ERK2-GFP fusion protein expressed in cells. This association
allows energy transfer to occur between the excited state Tb
fluorophore and GFP, leading to an increase in TR-FRET signal |
TR-FRET |
Life
Technologies |