Establishing a High-content Analysis Method for Tubulin Polymerization to Evaluate Both the Stabilizing and Destabilizing Activities of Compounds

Table 2: Comparison of potency values across three assay platforms.

Compound	Platform	Properties	EC50 / IC50 (µmM)	log EC50±S.E. / log IC50±S.E. (M) ^a	N ^b
Paclitaxel	Tubulin high-content	Increased tubulin polymerization	0.004	–8.4 ± 0.1	2
	Biochemical	Increased tubulin polymerization	0.010	–8.0 ± 0.3	1
	Cell-cycle	Increased G2 arrest	0.002	–8.6 ± 0.4	2
Nocodazole	Tubulin high-content	Decreased tubulin polymerization	0.244	–6.6 ± 0.1	2
	Biochemical	Decreased tubulin polymerization	2.292	–5.6 ± 0.3	1
	Cell-cycle	Increased G2 arrest	0.072	–7.1 ± 0.1	2

^a S.E. denotes standard error of the estimated values from non-linear dose-response regression analyses in a single curve or in a global fitting of data when there are two experiments

^b N = number of experiments