Fig. (2) High content imaging to quantitate the heterogeneity of reporter expression in individual spheroids using all three dimensions. A) Maximal projection images of spheroids where Z-stack images were taken at steps of 2.6 μm over a Z span of 240μm. Individual planes were combined in a maximal projection. High content output parameters shown across each focal plane of spheroids shown in B) The sum of fluorescence intensity across each plane, and C) The area of CK5Pro-GFP expression in μm2. Compound abbreviations are: RU486 (RU), Isotretinoin (RA), progesterone (P4), and DMSO is the vehicle control.