Fig. (12) Left: Dose response IC₅₀ values (M) of ~1,200 compounds which were designated Active in the Primary and Counter screen assays in EGFP-LC3-HeLa cells plotted vs. their cytotoxicity activity in the Secondary starvation assay in H1299 cells. So that they appear on the plot, compounds are set to 100 μM activity if inactive (red, n = ~450), and those with IC₅₀ values > 30 μM are set to 50 μM (n= ~50). For the two secondary assays in H1299 cells, compounds designated Active Specific (green) were compounds which gave IC₅₀ values in the starvation medium which were 3x or more lower than in the parallel assay in full growth medium (~400 compounds). Active Non-specific compounds (yellow) have similar IC₅₀ values in both formats. Right: Examples of compound dose responses obtained in the secondary assay (pink: starved; blue: full growth medium). (A): Active Non-specific, (B) and (C): Active Specific.