Table 2: Example Data for Filtering Compound Interference Using 4 Features

Compound % Pos (Norm) Icyt (Norm) Dmrk (Norm) Npass (Raw)
Negative Control (0.3% DMSO) -0.2 1.6 0.7 597
Positive Control (Wortmannin 33 nM) 101.3 -5.4 1.1 577
Fluorescent compound 94.6 56.1 42.9 631
GFP quenching compound 43.5 -73.8 -4.8 627
Very toxic compound 94.5 -48.3 -26.2 153
Somewhat toxic compound 107.2 -46.3 -17.0 551

‘Norm’ values are normalized to the mean of all plate wells; N Pass (Raw) is cell number in 4 fields of view