Table 1: Assay Optimization in 1536-well Format.

Cells/well (5µl) S/B EC50 (nM) Z’ factor
1,000 9.18 0.33 ± 0.032 0.76
1,500 7.13 0.55 ± 0.036 0.88
2,000 9.49 0.39 ± 0.038 0.76

Cells were plated 4 h prior to assay and stimulated with T3. Signal-to-background ratio (S/B) was calculated comparing the response of 4.6 μM T3 to DMSO controls. EC50 values were calculated from full dose response curves (n=3). Z’ factor was calculated using T3 EC100 (n=3).