Identification of Thyroid Hormone Receptor Active Compounds Using a Quantitative High-Throughput Screening Platform

Table 2: T₃ Concentration Optimization for Antagonist Mode Screening in 1536-well Format.

T₃ (nM)	S/B	CV (%)	Z’ factor
0.5	3.6	11.1	0.44
1	3.7	7.0	0.65

Cells were plated 4 h prior to assay and stimulated with T₃. Signal-to-background ratio (S/B) was calculated comparing the response of the respective T₃ concentrations to DMSO controls. Coefficient of variation (CV) and Z’ factor were calculated using the indicated T₃ concentrations (n=2).