Table 3: Screening Statistics for LOPAC and NTP Libraries.
Agonist Mode
Antagonist Mode
Library
CV (%)
S/B
Z’ factor
CV (%)
S/B
Z’ factor
NTP
9.21± 1.81
7.42± 0.51
0.77± 0.05
7.25± 3.77
4.71± 0.29
0.65± 0.08
LOPAC
11.04± 1.99
6.88± 0.54
0.82± 0.05
7.83± 1.27
3.37± 0.18
0.76± 0.04
Coefficient of variation (CV) calculated from control and low concentration plates. Signal-to-background ratio (S/B) and Z’ factor for agonist and antagonist mode determined for
100nM T3 and 1nM T3, respectively.