| Characteristics | HPLC | UPLC |
|---|---|---|
| Particle size | 3 to 5µm | Less than 2µm |
| Maximum backpressure | 300-400 bars | 1000 bars |
| Analytical column | C18 | UPLC BEH C18 |
| Column dimensions | 150 X 3.2 mm | 50 X 2.1 mm |
| Injection volume | 5µL | 2µL |
| Column temperature | 30 ° C | 65 °C |
| Total run time | 10 min. | 1.5 min |
| USP resolution | 3.2 | 3.4 |
| Plate count | 2000 | 7500 |
| Flow rate | 3.0 ml/min | 0.6ml/min |