Fig. (8) Nitrate and dissolved organic matter in the artificial seawater used for the tunic specimen. The absorbance at 220 nm (A220) and the integrated absorbance at 250 – 350 nm (ΣA250-350) are corresponding to the concentrations of nitrate and dissolved organic matter, respectively. The following samples were evaluated: No stimulus, for the specimen without the mechanical stimuli (n = 11); MS, for the specimen when the mechanical stimuli were given to it (n = 2); Post, for the specimen after the mechanical stimuli were given (n = 2); ASW, not used for the tunic specimen (n = 1). The regression line for all the samples is indicated (p < 0.05).