Fig. (2) Identification of a BRCA1 frame shift mutation in Kurdish Jewish women. Genomic DNA was purified from a Kurdish Jewish women and the 22 gene panel applied. The library was massive parallel sequenced using the Ion Torrent PGM and visualized using the Integrative Genomics Viewer (IGV) software. The BRCA1 c.224_227delAAAG is identified using the panel approach (A) and verified by Sanger sequencing (B). This mutation in Kurdish Jewish women is a deletion resulting in a frame shift from amino acid 75.