Inhibition of Renal Fibrosis and Glomerular Injury by Sacubitril/Valsartan, a Combination Angiotensin Receptor Blocker and Neprilysin Inhibitor, in a Salt-Sensitive Hypertensive Model Using Angiotensin 1 Receptor Knockout Mice: The Contribution of Non-Angiotensin Blocking Effects to Renal Protection

Fig. (5) TGF-β1 real-time PCR. We compared TGF-β1 mRNA by real-time PCR. After the treatment with aldosterone and 1% NaCl, TGF-β1 mRNA is significantly increased (p < 0.05), and Sac/Val; sacubitril/valsartan (low and high dose) significantly decreases TGF-β1 mRNA compared to valsartan (low and high dose) (p<0.05). NT, non-treatment; CON, Control; Val, Valsartan; Sac/Val, Sacubitril/Valsartan.