Open Medicine Journal




ISSN: 1874-2203 ― Volume 6, 2019
RESEARCH ARTICLE

Standardization and Detailed Aspects of Chopchinyadi Churna: A Potent Anti-Arthritic Medicine



Rashmi Saxena Pal*, Yogendra Pal, Pranay Wal, Ankita Wal
Department of Pharmacy, NH-2, Bhauti, PSIT, Kanpur, India

Abstract

Background:

Chopchinyadi Churna is a powdered Ayurvedic churna, commonly used for treating insect bite, rheumatoid arthritis, gout arthritis.

Objective:

The current research is oriented for the evaluation of ingredients and other aspects of Churna.

Materials and Methods:

The Churna was standardized as per the parameters of Ayurvedic Formulary of India for the organoleptic characters, microscopy, physicochemical, chromatographic, rheological properties and phytochemical screening for the detection of major phytoconstituents.

Results:

The parameters were found to be significant and offered future benefits for the advanced evaluation of Churṇa.

Conclusion:

Herbal based anti-arthritic medicine Chopchinyadi Churna has been evaluated on the basis of various parameters, which can serve as references for developing the pharmacopoeial standards.

Keywords: Chopchinyadi, Churna, Anti-arthritic, Medicine, Standardization, Phytoconstituents.


Article Information


Identifiers and Pagination:

Year: 2019
Volume: 6
First Page: 19
Last Page: 25
Publisher Id: MEDJ-6-19
DOI: 10.2174/1874220301906010019

Article History:

Received Date: 17/01/2019
Revision Received Date: 25/03/2019
Acceptance Date: 19/04/2019
Electronic publication date: 30/04/2019
Collection year: 2019

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© 2019 Pal et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Department of Pharmacy, NH-2, Bhauti, PSIT, Kanpur, India; Tel: 9129826459;
E-mail: rashmisaxenapal@gmail.com





1. INTRODUCTION

Plants are the richest source of pharmaceutical lead molecules and their contribution to the drug discovery process is remarkable. Many herbs and herbal medicines have been used since time immemorial to cure many disorders/diseases, including arthritis [1Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: Concept of ayurveda. Pharmacogn Rev 2014; 8(16): 73-80.[http://dx.doi.org/10.4103/0973-7847.134229] [PMID: 25125878] ]. In the traditional system of Indian medicine, combined extracts of individual plants are preferred rather than individual ones to achieve maximum therapeutic efficacy [2Jayakumar RV. Herbal medicine for type-2 diabetes. Int J Diabetes Dev Ctries 2010; 30: 111-2.[http://dx.doi.org/10.4103/0973-3930.66501] ]. Nature has gifted us enormous wealth in the form of herbal plants, distributed all over the world as medicinal agents for the treatment of various diseases [3Kalaria P, Gheewala P, Chakraborty M, Kamath J. A phytopharmacological review of Alstoniascholaris: A panoramic herbal medicine. IJRAP 2012; 3: 367-1.]. According to the WHO, 80% of the world’s population depends on herbal medicines for their primary health care requirements. Herbal medicines are irreplaceable and an important part of human society to combat diseases from a civilization [4Sudha K, Mathanghi SK. Traditional underutilized green leafy vegetables and its curative properties Int J Pharm 2012; 2: 786-93.].The medicinal properties of these herbal plants owe to the presence of chemical constituents that produce a desired physiological action on the body [5Singh V, Patel H, Suvagiya V, Singh K. Some traditionally used anti-arthritic herbs a review. Int Res J Pharm 2011; 2: 43-5.]. The preparation of a drug according to the ancient methods has minimized due to the commercialization of Ayurvedic pharmacy, therefore quality control is essential [6New Delhi: Govt of India, Ministry of Health and Family Welfare 1976.]. Chopchinyadi Churna is an Ayurvedic medicine, used in treating insect bite, rheumatoid arthritis, and gout, used commonly in North India [7 Chopchinyadichurna Benefits, Dosage, Ingredients, Side Effects. Ayurvedic Medicine Information ByDr JV Hebbar cited from. https://ayur medinfo.com/ 2012/ 05/ 29/chop chinyadi- churna- benefits- dosage- ingredients-side-effects/]. Standardization of a herbal formulation is essential in order to ensure the quality, purity, safety and efficacy of the constituent drugs. Chopchinyadichurna is a polyherbal formulation comprising 11 ingredients i.e. madhusnuhi(smilax china), vidanga (embeliaribes), triphala, sugar, cinnamon,tvakpippali (piper longum), lavanga (clove), akarakarabha (Anacyclus pyrethrum), kokilaksha (Asteracanthalongifolia), marica (piper nigrum), and sunthi (Zinziberofficinale). Smilax china L. is known as chobchini in Hindi, madhusnuhi in Sanskrit and china root in English. It shows medicinal properties as being anti-inflammatory, diuretic, anti-diabetic, antipsoriatic, and digestive [8Jayprakasam R, Ravit K, Athira K. AshaJyothi B. Quantitative determination of Diosgenin in polyherbal formulation and various extracts of smilax china linn using standard marker by validated analytical technique. Universal Journal of Pharmacy 2013; 02(04): 83-90.-11Rajesh B, Anupama S, Vikas A, Veerma R, Anil B. Pharmacognos- tical standardization, extraction and anti-diabetic activity of Smilax china L. rhizome. Asian J Tradit Med 2011; 6(5): 218-22.]. Embelia ribes Burm F., is also known as false black pepper or vidanga. It is a valuable medicinal plant exhibiting anthelmintic, carminative, antibacterial, antibiotic, hypoglycemic, and antifertility actions [12Syed A, Ramangdang R. Pharmacognosy of embelia ribes burm F IJ- RPC 1(4): 1236-50.2011; , 13Sudani RJ, Akbari BV, Vidyasagar G, Sharma P. Quantitative and chromatographic fingerprint analysis of embeliaribeschurna formulations by HPLC method. Int J Pharm Biol Arch 2011; 2(2): 611-8.]. All the other constituents of the formulation also have anti-arthritic potential. Though the contents of the formulation are rich enough to act against arthritis as most of them are potent anti-inflammatory agents. The present research focuses on the detailed aspects and complete standardization of Chopchinyadi churna composed of the constituents, which are used in the treatment of arthritic pains.

2. MATERIALS AND METHODS

Chopchinyadi churna was procured as shown in Fig. (1) from the local market of Kanpur. It contains eleven ingredients, which are mentioned in Table 1. All of the constituents present act as potent anti-arthritic agents.

Fig. (1)
Chopchinyadi churna.


2.1. Role of Individual Constituents as an Anti-Arthritic Agent

2.1.1. Chopchini

Smilax chinensis, belonging to the family Liliaceae, actively acts as an anti-diabetic, anti-inflammatory, antioxidant, anticancer, analgesic, inflammation agent [14Huang ZQ, Huang YR. Clinical application of single Smilax china L. on prolong life of the patients suffer from liver neoplasms Chinese National Folk Medicine Journal 2000; 45: 212-13., 15Khare C P. Indian medicinal plants An lllustrated dictionarySpringer science 2007; 79.]. The aqueous extract of Smilax china L. shows anti-inflammatory and anti-nociceptive activities [16Shu XS, Gao ZH, Yang XL. Anti-inflammatory and anti-nociceptive activities of Smilax china L. aqueous extract. J Ethnopharmacol 2006; 103(3): 327-32.[http://dx.doi.org/10.1016/j.jep.2005.08.004] [PMID: 16387460] ].

2.1.2. Triphala

An Indian traditional Ayurvedic herbal formulation Triphala, which is composed of amla, bahera and harde has already been evaluated for the antiarthritic effect on adjuvant-induced arthritis in mice [17Rasool M, Sabina EP. Antiinflammatory effect of the Indian Ayurvedic herbal formulation Triphala on adjuvant-induced arthritis in mice. Phytother Res 2007; 21(9): 889-94.[http://dx.doi.org/10.1002/ptr.2183] [PMID: 17533629] ]. Triphala, containing Terminalia chebula Retz., Terminalia bellerica Roxb. and Emblica officinalis , is used for treating bowel-related complications, inflammations and gastritis [18Kalaiselvan S, Rasool MK. The anti-inflammatory effect of triphala in arthritic-induced rats. Pharm Biol 2015; 53(1): 51-60.[http://dx.doi.org/10.3109/13880209.2014.910237] [PMID: 25289531] ]. Ayurveda doctors recommend this formulation to relieve symptoms of inflammatory joint problems like rheumatoid arthritis, psoriatic arthritis and gout [19 Psoriasis management.Triphala for Psoriasis and Psoriatic Arthritis.by Ashish Agarwal November 6, 2017.cited from http://www. psoria sisself management.com /detoxification-ideas/triphala-leaky-gut-psoriasis-psoriatic-arthritis/].

Table 1
Constituents of chopchinyadi Churna.


2.1.3. Pippali

The root of this plant is known as Pippali Mula in Ayurveda and its fruits found in the form of the spike are mainly used for Rasayana purpose. Several pharmacological activities like anti-ulcer, anti- amoebic, anti-oxidant and anti-inflammatory activities have been reported for the fruit of this plant [20Warrier PK, Nambiar VP, Raman KC. 1995; 4: 290., 21Dahanukar SA, Karandikar SM. Evaluation of anti-allergic activity of Piper longum. Indian Drugs 1984; 21: 377-83.].

2.1.4. Maricha

Maricha is also botanically known as piper nigrum.The main constituent of it is piperine, which shows anti- inflammatory, antinociceptive and antiarthritic effects.Black pepper or Piper nigrum is commonly used as a spice, but it also has multiple uses as a medicine, a preservative, and in perfumery. Its extract from the plant is rich in active phenolic component, piperine [22Srinivasan K. Black pepper and its pungent principle-piperine: A review of diverse physiological effects. Crit Rev Food Sci Nutr 2007; 47(8): 735-48.[http://dx.doi.org/10.1080/10408390601062054] [PMID: 17987447] ]. Its anti-inflammatory activities have been exhibited in rat models of carrageenan-induced paw edema [23Mujumdar AM, Dhuley JN, Deshmukh VK, Raman PH, Naik SR. Anti-inflammatory activity of piperine. Jpn J Med Sci Biol 1990; 43(3): 95-100.[http://dx.doi.org/10.7883/yoken1952.43.95] [PMID: 2283727] ]. Constituents of the piper species are responsible for in vitro inhibitory activity against the enzymes responsible for leukotriene and prostaglandin biosynthesis [24Stöhr JR, Xiao PG, Bauer R. Constituents of Chinese Piper species and their inhibitory activity on prostaglandin and leukotriene biosynthesis in vitro. J Ethnopharmacol 2001; 75(2-3): 133-9.[http://dx.doi.org/10.1016/S0378-8741(00)00397-4] [PMID: 112978 43] ].

2.1.5. Clove

Clove contains essential oil, which possesses anti-inflammatory and anti-arthritic properties [25Han X, Parker TL. Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharm Biol 2017; 55(1): 1619-22.[http://dx.doi.org/10.1080/13880209.2017.1314513] [PMID: 284077 19] ]. At a lower concentration of 0.03% v/v, the anti-inflammatory activity of eugenol, which is the important constituent of its oil has been demonstrated in human gingival fibroblast and pulp cells [26Prashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Prolif 2006; 39(4): 241-8.[http://dx.doi.org/10.1111/j.1365-2184.2006.00384.x] [PMID: 168723 60] , 27Koh T, Murakami Y, Tanaka S, Machino M, Sakagami H. Re-evalua- tion of anti-inflammatory potential of eugenol in IL-1β-stimulated gingival fibroblast and pulp cells. In Vivo 2013; 27(2): 269-73.[PMID: 23422489] ].

2.1.6. Akarakarabha

Anacyclus pyrethrum is widely used in Moroccan traditional medicine. It is active against inflammatory and painful diseases. Antinociceptive and anti-inflammatory activities have been exhibited by the aqueous and methanol extracts of roots of Anacyclus pyrethrum [28Manouze H, Bouchatta O, Gadhi AC, Bennis M, Sokar Z, Ba-M’hamed S. Anti-inflammatory, antinociceptive, and antioxidant activities of methanol and aqueous Extracts of Anacyclus pyrethrum Roots. Front Pharmacol 2017; 8: 598.[http://dx.doi.org/10.3389/fphar.2017.00598] [PMID: 28928658] ]. N-isobutyldienediynamide and polysaccharides are present in its roots [29Crombie L. Isolation and structure of an n-isobutyldienediynamide from pellitory (Anacyclus pyrethrum dc.). Nature 1954; 174: 832-3.[http://dx.doi.org/10.1038/174832a0] -32Boonen J, Sharma V, Dixit VK, Burvenich C, De Spiegeleer B. LC-MS N-alkylamide profiling of an ethanolic Anacyclus pyrethrum root extract. Planta Med 2012; 78(16): 1787-95.[http://dx.doi.org/10.1055/s-0032-1315371] [PMID: 23047251] ]. It treats rheumatic arthritis by increasing circulation [33Usmani A. Pharmacognostic and phytopharmacology study of Anacyclus pyrethrum: An insight. J Appl Pharm Sci 2016; 6(03): 144-50.[http://dx.doi.org/10.7324/JAPS.2016.60325] ].

2.1.7. Asteracantha Longifolia

also known as askokilasha, helps in alleviating the symptoms of rheumatoid arthritis [34Thankamma A. Rheumatoid arthritis and astercantha longifolia. Anc Sci Life 1999; 18(3-4): 247-9.[PMID: 22556897] ]. It is rich in phytoconstituents as alkaloids, flavonoids, terpenoids, essential oil. It has been used in the traditional systems of medicine. Many pharmacological studies have been performed on the plant signifying its antioxidant and analgesic potential [35Imtiyaz S, Rahman K. Asteracanthalongifolia Linn: An Overview American Journal of PharmTech Research 2013; 3(1): 15-26.].

2.1.8. Zingiber Officinalis

Ginger is primarily known as curative [36Barnes PM, Powell-Griner E, McFann K, Nahin RL. Complementary and alternative medicine use among adults: United States, 2002. Adv Data 2004; 343(343): 1-19.[http://dx.doi.org/10.1016/j.sigm.2004.07.003] [PMID: 15188733] ]. It has also been used medicinally as an anti-inflammatory agent since antiquity [37Palatty PL, Haniadka R, Valder B, Arora R, Baliga MS. Ginger in the prevention of nausea and vomiting: A review. Crit Rev Food Sci Nutr 2013; 53(7): 659-69.[http://dx.doi.org/10.1080/10408398.2011.553751] [PMID: 23638927] -39Grzanna R, Lindmark L, Frondoza CG. Ginger an herbal medicinal product with broad anti-inflammatory actions. J Med Food 2005; 8(2): 125-32.[http://dx.doi.org/10.1089/jmf.2005.8.125] [PMID: 16117603] ]. Gingerols, the phenolic compounds are responsible for its pungent taste and usage in treating inflammatory disorders as arthritis [40Chrubasik S, Pittler MH, Roufogalis BD. Zingiberis rhizoma: A comprehensive review on the ginger effect and efficacy profiles. Phytomedicine 2005; 12(9): 684-701.[http://dx.doi.org/10.1016/j.phymed.2004.07.009] [PMID: 16194058] ]. The potent anti-arthritic effects of the extracts of ginger have been proven in an experimental model of rheumatoid arthritis. However, crude extracts containing both gingerols as well as essential oils have been found to be even more potent in inhibiting joint swelling as compared to gingerols alone [41Funk JL, Frye JB, Oyarzo JN, Timmermann BN. Comparative effects of two gingerol-containing Zingiber officinale extracts on experimental rheumatoid arthritis. J Nat Prod 2009; 72(3): 403-7.[http://dx.doi.org/10.1021/np8006183] [PMID: 19216559] ].

2.1.9. Embelia Ribes

It is commonly known as Vidanga. It is used as a powerful anthelmintic in Ayurveda [42Hördegen P, Cabaret J, Hertzberg H, Langhans W, Maurer V. in vitro screening of six anthelmintic plant products against larval Haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay. J Ethnopharmacol 2006; 108(1): 85-9.[http://dx.doi.org/10.1016/j.jep.2006.04.013] [PMID: 16725288] ]. It has also been evaluated to have antifertility action [43Gupta S, Sanyal SN, Kanwar U. Antispermatogenic effect of embelin, a plant benzoquinone, on male albino rats in vivo and in vitro. Contraception 1989; 39(3): 307-20.[http://dx.doi.org/10.1016/0010-7824(89)90063-2] [PMID: 2714091] ]. Analgesic property has been reported for embelin and its derivatives [44Zutshi U, Johri RK, Atal CK. Possible interaction of potassium embelate, a putative analgesic agent, with opiate receptors. Indian J Exp Biol 1989; 27(7): 656-7.[PMID: 2561116] ]. It is used as an anti-inflammatory drug in the treatment of rheumatism and fever [45Kapoor VK, Chawla AS, Kumar M, Kumar P. Anti-inflammatory agent in Indian Laboratories. Indian Drugs 1983; 30: 481-8.].

2.1.10. Cinnamomum Zeylanicum

Cinnamon is used for its aroma in the essence industries. Due to its fragrance, it is applicable for use in different varieties of foodstuffs, perfumes, and medicinal products [46Huang T-C, Fu H-Y, Ho C-T, Tan D, Huang Y-T, Pan M-H. Induction of apoptosis by cinnamaldehyde from indigenous cinnamon CinnamomumosmophloeumKaneh through reactive oxygen species production, glutathione depletion, and caspase activation in human leukemia K562 cells. Food Chem 2007; 103(2): 434-43.[http://dx.doi.org/10.1016/j.foodchem.2006.08.018] ]. Cinnamon bark is rich in ingredients as procyanidins and catechins [47Nonaka G-I, Morimoto S, Nishioka I. Tannins and related compounds. Part 13. Isolation and structures of trimeric, tetrameric, and pentamericproanthicyanidins from cinnamon. J Chem Soc, Perkin Trans 1 1983; 2139-45.[http://dx.doi.org/10.1039/p19830002139] ]. The components of procyanidins include both procyanidin A and B [48Anderson RA, Broadhurst CL, Polansky MM, et al. Isolation and characterization of polyphenol type-A polymers from cinnamon with insulin-like biological activity. J Agric Food Chem 2004; 52(1): 65-70.[http://dx.doi.org/10.1021/jf034916b] [PMID: 14709014] -50Tanaka T, Matsuo Y, Yamada Y, Kouno I. Structure of polymeric polyphenols of cinnamon bark deduced from condensation products of cinnamaldehyde with catechin and procyanidins. J Agric Food Chem 2008; 56(14): 5864-70.[http://dx.doi.org/10.1021/jf800921r] [PMID: 18558701] ]. Therefore it has been used as an anti-inflammatory agent [51Chao LK, Hua K-F, Hsu H-Y, Cheng S-S, Liu J-Y, Chang S-T. Study on the antiinflammatory activity of essential oil from leaves of Cinnamomum osmophloeum. J Agric Food Chem 2005; 53(18): 7274-8.[http://dx.doi.org/10.1021/jf051151u] [PMID: 16131142] -53Tung Y-T, Yen P-L, Lin C-Y, Chang S-T. Anti-inflammatory activities of essential oils and their constituents from different provenances of indigenous cinnamon (Cinnamomum osmophloeum) leaves. Pharm Biol 2010; 48(10): 1130-6.[http://dx.doi.org/10.3109/13880200903527728] [PMID: 20815702] ].The major pharmacological uses of cinnamon bark are due to its anti-inflammatory and anti-microbial properties [54Oussalah M, Caillet S, Lacroix M. Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157: H7 and Listeria monocytogenes. J Food Prot 2006; 69(5): 1046-55.[http://dx.doi.org/10.4315/0362-028X-69.5.1046] [PMID: 16715803] -57Cabello CM, Bair WB III, Lamore SD, et al. The cinnamon-derived Michael acceptor cinnamic aldehyde impairs melanoma cell proliferation, invasiveness, and tumor growth. Free Radic Biol Med 2009; 46(2): 220-31.[http://dx.doi.org/10.1016/j.freeradbiomed.2008.10.025] [PMID: 19000754] ].

2.2. Phytochemical Screening

Plant extracts when tested for preliminary phytochemical screening, the presence of phytoconstituents as alkaloids, carbohydrates and reducing sugars, glycosides, proteins and amino acids, steroids and triterpenoids, tannins, flavonoids, fixed oils, volatile oils were detected. The methanolic extracts of the formulation were concentrated for further use.

2.3. Tests for Volatile Oils

The non-permanent staining of filter papers, along with the characteristic odour indicated the presence of volatile oils.

2.4. Tests for Alkaloids

Methanolic extracts of all the drugs were acidified and then filtered to obtain the filtrate to carry out further tests.

2.5. Hager’s Test

A yellow precipitate was formed with it.

2.6. Tests for Carbohydrates and Reducing Sugars:

2.6.1. Molisch’s Test

To drug extracts, a few drops of alcoholic α-naphthol solution, followed by concentrated sulphuric acid were added. A purple ring at the junction of two liquids indicated the presence of carbohydrates.

2.6.2. Fehling’s Test

Fehling A and Fehling B solutions were mixed with equal volumes of the filtrates and heated for 5 minutes. First yellow, followed by brick red precipitate indicated the presence of reducing sugars.

2.7. Tests for Glycosides

2.7.1. Foam Test

Drugs in small quantities were shaken vigorously with water. The formation of persistent foam indicated the presence of saponin glycosides.

2.8. Tests for Phenolic Compounds and Tannins

With 5% Ferric chloride solution, they gave a deep blue-black color [58Sane RT. Standardization, quality control and GMP for herbal drug. Indian Drugs 2002; 39(3): 184-90., 59Khandelwal KR. Practical Pharmacognosy, Techniques and Experiments 12th ed. 12th ed.1996.].

2.9. Organoleptic Evaluation

Dried powdered churna was taken in order to examine the macroscopic features of the formulation (Fig. 2). The morphological features were determined with the use of a simple microscope [60Trease and Evans. Pharmacognosy 16th.1997.].

Fig. (2)
Churna sample.


2.10. Microscopic Evaluation

Coarsely powdered, air-dried samples were used for the purpose of microscopic evaluation. These were then treated with reagents like phloroglucinol and hydrochloric acid, followed by treatment with clearing agents, such as lactophenol and chloral hydrate in order to remove the impurities adhered. Then finally, they were mounted in glycerine. Glycerine, being an emollient, saves the sample from drying. Different cell components were studied as in Figs. (3a and 3b) per the standard methods with the help of different freshly prepared reagents [61Sass JE. Elements of Botanical Micro Technique 1940; 222.-64Pal RS, Pal Y, Wal P, Wal A. Pharmacognostic Evaluation of Roots of Benincasa Hispida (Thunb.) Cogn.(Cucurbitaceae) The Open Plant Science Journal 2018; 11: 1-6.].

Fig. (3)
Microscopy of Churna.


2.11. Physico-Chemical Evaluation

Physicochemical standards as ash values, moisture content and extractive values were determined as per official methods and procedures.

2.12. Thin Layer Chromatography

A solvent system was used i.e toluene:ethyl acetate (9:3). Plates with the spotted sample were kept in the TLC chamber. Then, the chamber was closed with a lid. After spot development, the plates were removed and dried. Then sample spots were visualized in a UV light chamber (Fig. 4).

2.13. Rheological Evaluation

Coarsely powdered crude drug was screened for different parameters. Bulk density, angle of repose, compressibility, and Hausner’s ratio were calculated according to standard methods.

2.13.1. Bulk Density

10g of the powdered drug was taken in a graduated measuring cylinder and tapped on a wooden surface. Bulk density = weight taken / Bulk volume Tapped density = weight of drug taken / volume (tapped).

2.13.2. Angle of Repose

The angle of repose was determined by using the funnel method. The powderwas made to flow through a funnel fixed on a stand to form a heap. The height and the radius gave the angle of repose. The angle of repose tanθ = h/r θ = tan-1 (h/r). Where, h = height of heap, r = radius of the heap.

2.13.3. Compressibility / Carr’s Index

It is: Bulk density (Tapped) − Bulk density (Untapped)/ Bulk density (Tapped) Hausner’s Ratio. The formula used to determine Hausner’s ratio is Bulk density (Tapped) x 100 /Bulk density (untapped) [65Lachman L, Lieberman HA, Kanig JL. The theory and practice of industrial pharmacy 3rd ed. 3rd ed.1987; 183-316., 66Aulton ME. Pharmaceutics, the science of dosage forms designs 2nd ed. 2nd ed.2002; 205-21.]. In spectral analysis, the churna was subjected to spectral techniques as Infra –red and ultraviolet spectroscopy [67Mukherjee PK. Quality control of Herbal drugs an approach to evaluation of botanicals 1st ed. 1st ed.2002.-69The Ayurvedic Pharmacopoeia of India, Government of India, Ministry of Health and family welfare, Department of AYUSH 2001; 48-9: 173-4.].

3. RESULTS

Phytochemical analysis revealed the presence of alkaloid, tannins (due to the presence of amla, bahera, myrobalans in the form of triphala) reducing sugars, volatile oil (due to the presence of ginger, clove and piper species)and the absence of carbohydrate, saponins. It has been concluded that Churna is free from heavy metals. From the all above values, efficacy of the Churna is revealed (Table 2).

Table 2
Phytochemical screening of Churna.


Botanical parameters revealed that Churna was light brown, moderately fine powder, odour- characteristic, taste- slightly sweet (Table 3).

Table 3
Organoleptic evaluation of Churna.


Results of quantitative analysis for loss on drying at 1050 C, total ash, acid insoluble ash, water soluble ash, alcohol soluble extractives and water soluble extractive were calculated and results are shown (Table 4). Ash value helps to assure the authenticity and purity of the drug. The loss on drying indicates the amount of moisture content present in the drug. Moisture content was found to be 8% w/w. The less value of moisture content prevents bacterial, fungal or yeast growth. The obtained values as water soluble and alcohol soluble indicate the amount of active constituent in the given amount of drug when extracted with the respective solvent. The Rf value was found to be 0.63.

Fig. (4)
TLC of Churna.


Table 4
Physico-chemical evaluation of Churna.


Rheological properties of churnas shown in Table 5 like bulk density, tapped density, Hausner’s ratio and Carr's index indicate very poor flowability and the angle of repose indicates Poor-must agitate, with vibrate flow property.

3.1. Spectral Analysis of Churna

UV spectrophotometry revealed the absorbance 0.173 at 260 nm. Infrared spectroscopy revealed the presence of peaks at 1700 cm- and 3400 cm-, indicating the presence of keto and aldehyde groups containing phytoconstituents in the formulation (Fig. 5).

4. DISCUSSION

Phytochemical screening of churna showed the absence of carbohydrates and saponins. It revealed the presence of tannins, reducing sugars, volatile oil and alkaloids. Morphology discussed the light brown, slightly sweet taste of it. The moisture content was found to be 8% w/w. The less value of moisture content prevents bacterial, fungal or yeast growth. The extractive values as water soluble and alcohol soluble indicate the amount of active constituent in the given amount of drug when extracted with the respective solvent. Powder on its flow study showed that it has a very poor flowability and the angle of repose indicates poor must agitate, with vibrate flow property. Spectral analysis indicated the presence of keto and aldehyde groups containing phytoconstituents in the formulation [70Haslam E. Natural polyphenols (vegetable tannins) as drugs: Possible modes of action. J Nat Prod 1996; 59(2): 205-15.[http://dx.doi.org/10.1021/np960040+] [PMID: 8991956] , 71Jones GA, Mc Allister T, Muir AD, Chang KJ. Appl Environ Microbiol Lett 1994; 46: 223-7.].

CONCLUSION

Various evaluation parameters like physicochemical standards as total ash, acid insoluble ash, water and alcohol-soluble extractive values, loss on drying, microscopy, spectroscopy, phytochemical analysis, flow properties, and morphological features were evaluated. From their results, it was revealed that the formulation of Churna is effective with uniform characteristics of an ideal Churna. It is beneficial, effective and economic as well. The efficacy of the Churna can be proved by the pharmacology which suggests the future scope of R & D. Though the contents of the formulation are rich enough to act against arthritis as most of them are potent anti-inflammatory agents. The study shows that the ingredients of churna presentare in accord with the prescribed guidelines of the WHO. All these observations are not mentioned in the standard literature, which could be helpful in authentication and assurance of Churnas. The result of the present study will help to set standards as a reference monograph in the process of drug formulation. Ayurvedic Churna has been standardized by the intervention of modern scientific quality control measures in the traditional preparation described in classical texts. Pharmacognostic characters established for the raw materials could be employed as Q.C. standards for evaluating cccstheir identity and can be used for routine analysis.

Table 5
Rheological evaluation of Churna.


Fig. (5)
IR Spectra of Churna.


ETHICAL APPROVAL AND CONSENT TO PARTICIPATE

Not applicable.

HUMAN AND ANIMAL RIGHTS

No animals/humans were used for studies that are the basis of this research.

CONSENT FOR PUBLICATION

Not applicable.

AVAILABILITY OF DATA AND MATERIALS

The authors confirm that data supporting the findings of this research are available within the article.

FUNDING

None.

CONFLICT OF INTEREST

The authors declare no conflict of interest, financial or otherwise.

ACKNOWLEDGEMENTS

Declared none.

REFERENCES

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