Table 2: HPLC methods for Thiocolchicoside.

Sr. No.	Name of drug	Sample matrix	Mobile phase composition	Detection (λ max) nm	Linearity	Rt (in. min)		Ref.
1	THC+GF	Tablet MIX-I	Methanol : 0.035 M phosphate buffer (50:50, v/v) pH 4.5	400	THC-0.2 – 2 µg/mL GF and FN -20–200 µg/mL	THC-2.56 GF-4.5	Good resolution with low Rt	[31]
	THC+ FN	Tablet MIX-II	Methanol: 0.03M phosphate buffer (70:30, v/v), pH 4			THC-3.84 FN-5.88
2	THC+ACE	Tablets	Acetonitrile: Water: Methanol (70:20:10, v/v)	260	THC-4 - 20 µg/mL ACE-40-200µg/mL	THC-3.36 ACE-4.12	Low Rt and Robust	[32]
3	THC+ACE	Tablets	Acetonitrile: Water: 0.025M pot. dihydrogen orthophosphate buffer (pH adjusted to 3.0 with OPA) (70:10:20, v/v)	260	THC-1-6 µg/mL ACE-25-150µg/mL	THC-2.70 ACE-4.76	Simple and sensitive	[33]
4	THC+ACE	Tablets	Phosphate buffer :Acetonitrile (40:60 v/v)	261	THC 2 - 12 µg/mL ACE 25-150µg/mL	THC-2.17 ACE-4.80	Simple mobile phase	[34]
5	THC+ACE	Tablets	Acetonitrile: buffer of pH 6 (42:58,v/v)	261	ACE 25-125 μg/mL THC 1-6 μg/mL	THC-4.15 ACE-4.88	Low resolution	[35]
6	THC+DCF	Capsules	Acetonitrile: Methanol: Water (35:15; 50, v/v), pH adjusted to 3.5 with orthophosphoric acid.	286	THC 10-100 μg/mL DCF- 20-100 μg/mL	THC-3.3 DCF-4.0	Low resolution	[36]
7	THC+DCF	Tablet	Water (pH 9.2adjusted with di- Potassium hydrogen Phosphate) (60: 40, v/v)	223	THC 50-150 μg/mL DCF- 50-150 μg/mL	DCF-3.229 THC-4.999	Low resolution	[37]
8	THC+LOR	Tablets	Methanol: THF: acetate buffer (60: 10: 30, v/v); pH adjusted to 5.5 with glacial acetic acid	250	LOR 0.2 to 80μg/mL THC 0.1 to 40μg/mL	LOR-.4.08 THC- 3.36	Low resolution	[38]
9	THC+LOR	Tablet	Methanol: Acetate buffer (PH-4.6) :THF(50:35:15, v/v)	375	LOR -4-100μg/mL THC-2-50μg/mL	LOR-.400 THC- 2.92	Complex mobile phase	[39]
10	THC+LOR	Tablet	Ammonium Dihydrogen Phosphate buffer (pH 7.3 with TEA): Methanol (45:55,v/v)	290	LOR-0.24 - 120µg/mL THC-0.23 - 120µg/mL	LOR -9.40 THC-2.96	Time consuming	[40]
11	THC+LOR	Tablet	Sodium Phosphate buffer( pH 6.8 adjusted with NaOH): ACN (35:65% v/v)	298	LOR - 1- 50 μg/mL THC-5 - 25 μg/mL	LOR -4.50 THC -3.40	Low resolution	[41]
12	THC+KET	Tablet	Acetonitrile: Water: Phosphate buffer (pH 3.0) (60:30:10, v/v)	260	THC-4-20µg/mL KET-20-100µg/mL	THC-2.70 KET-4.90	Low resolution	[42]
13	THC+KET	Tablet	Acetonitrile: Water (60:40,v/v)	300	KET-80-120μg/mL THC-6.4-9.6μg/mL	THC-3.7± 0.1 KET-7.90±0.1	Economical and good resolution	[43]
14	THC+DXKET	Tablet	Methanol: Sodium acetate buffer (pH 5 with Glacial acetic acid) (70:30, v/v)	265	DXKET-5-30µg/mL THC-1 –10 µg/mL	THC-3.0133 DXKET 6.013	Economical And well separation	[44]
15	THC+DXKET	Tablet	Methanol: Phosphate buffer (pH adjusted to 4.5 with OPA) (65:35 v/v)	260	THC 4-24 µg/mL DXKET-5-30 µg/mL	THC-3.02 DXK 8.91	Economical and well separation	[45]
16	THC+ETR	Tablet	Trifluoroacetic acid buffer (pH 2.6): Acetonitrile (75:25, v/v)	220	ETR 20-160µg/mL THC 2 -16 µg/mL	ETR-6.6 THC-3.1	Simple	[46]
17	THC+ETR	Tablet	1 mL TFA in 2 liter milli-Q water) and Acetonitrile (75:25 v/v)	258	ETR 1.598-90µg/mL THC 4.95-12µg/mL	THC-3.37 ETR-8.62	Simple	[47]
18	THC+ETR	Tablet	Phosphate buffer (pH 6, adjusted with orthophosphoric acid) and Methanol (30:70 v/v)	255	ETR 40-80 µg/mL THC 2-6 µg/mL	THC-2.50 ETR-4.60	Robust	[48]
19	THC+ETD	Tablet	Methanol and Phosphate buffer pH 6, (85:15 v/v)	259	ETD 16-96 µg/mL THC 4-24 µg/mL	ETD4.39±0.10 THC3.52±0.10	Simple mobile phase but less separation	[49]
20	THC+ETD	Tablet	Acetonitrile: 20mM potassium dihydrogen phosphate buffer (65:35 v/v)	257	ETD 25-150 µg/mL THC 0.5-10 µg/mL	THC-2.240 ETD-7.141	Robust, Good resolution	[50]
21	THC+ETD	Tablet	Acetonitrile: Potassium dihydrogen phosphate buffer (pH 3.0) (50:50, v/v)	255	ETD 100-600µg/mL THC 1-6 µg/mL	ETD-4.27 THC-2.6	high linearity values	[51]
22	THC+PCM	Tablet	Potassium Dihydrogen phosphate: Methanol (40:60, v/v)	247	PCM 250-750µg/mL THC 1-3µg/mL	PCM-3.27 THC-5.50	Economical with low Rt	[52]
23	THC+ ACE+ PCM	Tablet	Acetonitrile: Water (30: 70, v/v)	263	PCM-50-175 µg/mL ACE-10-35 µg/mL THC-0.40-1.4 µg/mL	PCM- 2.51 THC-3.55 ACE -5.20	Simple and economical	[53]
24	THC+ ACE+ PCM		Buffer of pH 6.5 and Acetonitrile in Gradient elution.	300	PCM 1250-3750 µg/mL, THC 20-60 µg/ mL ACE 250-750 µg/ mL	PCM- 2.70 THC-3.95 ACE -9.91	Well resolution	[54]