Table 3: HPLC chromatographic columns and optimized analytical parameters.

Sr. No.	Name of drug	Column	Internal diameter and Partical Size	Temp.	Detector	Flow rate	Mode of analysis	Diluents	Ref.
1	THC+GF	C18 (Waters symmetry)	i.d.-250×4.6mm; 5-µm	RT	UV detector	1mL/min	Isocratic	Methanol	[31]
	THC+FN
2	THC+ACE	C18 (Intersil)	250 mm x 4.6 mm, 20-µm	RT	Variable wavelength detector	1mL/min	Gradient	Methanol	[32]
3	THC+ACE	C18(Thermo)	i.d-250x4.6 mm;5-µm	Ambient	UV detector	1mL/min	Isocratic	M.P	[33]
4	THC+ACE	C18 (Waters symmetry)	i.d.-150×4.6mm; 5-µm	RT	UV detector	1mL/min	Isocratic	Methanol	[34]
5	THC+ACE	C18 (Thermo Hypersil BDS)	i.d.-250X4.6 mm; 5µm	RT	diode array detector	0.6mL/min.	Isocratic	MP	[35]
6	THC+DCF	C18 (Intersil)	i.d.-250 x 4.6 mm;5µm	RT	Variable wavelength detector	1mL/min	Gradient	Water	[36]
7	THC+DCF	C18 (Waters Symmetry)	i.d.250X4.6 ;5µm	30oC	PDA detector	1mL/min	Isocratic	M.P	[37]
8	THC+LOR	C18 (Waters Symmetry)	i.d.-250 mm × 4.6 mm;5.0 µm	50 0C	PDA detector	0.75mL/min	Isocratic	MP	[38]
9	THC+LOR	C18(Varian)	i.d.-250 mm x 4.6 mm ; 5 µm	25± 30C.	UV detector	0.5 mL/min	isocratic	Methanol	[39]
10	THC+LOR	C18 (Inertsil ODS 3V)	i.d.-250 x 4.6 mm; 5 µm	30ºC	PDA detector	1.5 mL/min	isocratic	MP	[40]
11	THC+LOR	C8 (X terra) ,	i.d.-4.6 x 250mm; 5 µm,	Ambient	UV detector	1mL/min	isocratic	MP	[41]
12	THC+KET	C18 (Thermo scientific)	i.d.-250×4.6 mm; 5μm	25 ±1 oC	UV detector	1mL/min	Isocratic	MP	[42]
13	THC+ KET	C18	i.d.-150 x 4.6 mm; 5μm	RT	UV detector	1mL/min.	Isocratic	MP	[43]
14	THC+ DXKET	C18 (HS, HiQ sil)	i.d.-250 x 4.6 mm; 5μm	Ambient	PDA detector	1mL/min.	Isocratic	MP	[44]
15	THC+DXKET	RP-18e (Purosphere STAR)	i.d.-250× 4mm ;5μm	RT	UV detector	1.0 mL/min.	Isocratic	MP	[45]
16	THC+ETR	C-18 (BDS Hypersil)	i.d.-250 X4.6 mm; 5-µm.	Ambient	UV detector	1.5 mL/min	Isocratic	MP	[46]
17	THC+ETR	C18 (RP-select B Lichrospher)	i.d.-250 x4.6mm;5 µm	---	PDA	1.5 mL/min	Isocratic	MP	[47]
18	THC+ETR	C18 (InertSil ODS-3)	i.d.-250x4.6 mm; 5µm	--	UV detector	1.2 mL/min	Isocratic	MP	[48]
19	THC+ETD	C-18 (Phenomenex)	i.d.-250 mm × 4.60 mm; 5 µm	--	UV detector	0.8mL/ min	Isocratic	MP	[49]
20	THC+ETD	C18 (HiQ sil HS)	i.d-250 x 4.6 mm; 5 µm	Ambient	UV detector	1mL/min	Isocratic	MP	[50]
21	THC+ETD	C18 (Symmetry)	i.d.-150X4.6 mm ;5µm	25 ±10C	UV detector	1.0 mL/min	Isocratic	Methanol	[51]
22	THC+ PCM	C18 (BDS hypersil)	i.d.-250mm × 4.6mm, 5µm	Ambient	UV detector	1.0 mL/min	Isocratic	MP	[52]
23	THC+ACE+PCM	C18 (HiQ Sil)	i.d.-250 mm × 4.6 mm, 5.0 µm	Ambient	UV detector	1.0 mL/min	Isocratic	Methanol	[53]
24	THC+ ACE+ PCM	C18 (inertsil ODS)	i.d-150x4.6mm ;5 µm	Ambient	PDA detector	1.0 mL/min	Gradient	MP	[54]