Table 4: HPTLC methods for determination of thiocolchicoside.

Sr.
No.
Name of Drug Sample matrix Mobile phase composition Detection (λmax) nm Linearity Rf Comments Ref.
1 THC+DCF Capsule Toluene: Ethyl acetate: Methanol (5:3:2 v/v) 285 50-300 ng/band for both THC-0.17
DCF-0.53
Simple and economical [55]
2 THC+DCF Capsule Toluene: Acetone: Methanol: Formic acid (5:2:2:0.01 v/v/v/v) 280 THC 160-800 ng/band
DCF 1000-5000 ng/band
THC-0.29±0.02 DCF-0.71±0.02 Complex mobile phase with high linearity values [56]
3 THC+ACE Tablet Toluene: Ethyl acetate: Methanol: Glacial acetic acid (4: 6: 2: 0.5 v/v). 255 THC 6–21 ng/band
ACE 10-35 ng/band
THC 0.16
ACE 0.79
High resolution [57]
4 THC+ACE Tablet Methanol:Chloroform:
Water 9.6:0.2:0.2 v/v)
254 30–180 ng/band THC 750–4500 ng/band
ACE
THC 0.70 ± 0.05 ACE 0.83 ± 0.05 Solvents with diverse polarity [58]
5 THC+ACE Methanol: Chloroform: Water (9.6: 0.2: 0.2 v/v) 254 THC 30-180 ng/band ACE 750-4500 ng/band THC 0.70 ± 0.05
ACE 0.83 ± 0.05
Low resolution [59]
6 THC+DXKET Tablet Toluene: Methanol: Ethyl Acetate (6: 2.5: 0.5, v/v) 280 THC-100-800 ng/band
DXKET-600-4800 ng/band
THC 0.33± 0.011
DXKET 0.61 ± 0.007
High linearity range [60]
7 THC+DXKET Tablet Toluene: Ethyl acetate: Methanol (5:3:2 v/v) 286 THC-50-350 ng/band
DXKET-100-700 ng/band
THC 0.10
DXKET 0.40
Low Rf of THC (not acceptable) [61]
8 THC+LOR Tablet Methanol:Chloroform:
Water (9.6:0.2:0.2 v/v)
377 THC 30-180 ng/band
LOR 60-360 9ng/band
THC 0.58±0.05
LOR 0.85±0.05
Water not sutable as solvent for NP [62]
9 THC+ETR Tablet Ethyl acetate: Methanol
(8 :2 v/v)
290 THC 100–500 ng/band ETR 50–250 ng/band THC 0.17
ETR 0.70
Low Rf value for THC [63]