| Sr.No. | Name of Drug | Sample matrix | Mobile phase | Detection (λ max) nm | Linearity | Rt/Rf | Comments | Ref. |
|---|---|---|---|---|---|---|---|---|
| 1 | THC | Capsule | Acetonitrile: Water (70:30) | 286 | THC- 0-10μg/mL | 3.35 min. | Simple | [72] |
| 2 | THC | Capsule | Acetonitrile: Phosphate Buffer pH 3.5, (70:30 % v/v) | 260 | 80-120 % of THC label claim | 2.24 min. | All parameters of forced degradation are not explored | [73] |
| 3 | THC | Capsule | Methanol:Water (70 : 30 v/v) | 377 | 100–600 ng per band. | 0.60 ± 0.02 | Simple and economical | [74] |
| 4 | THC+ETD | Tablet | Acetonitrile: Potassium dihydrogen phosphate buffer pH 6.8 (70:30% v/v) | 260 | THC-4.012 - 12.03 μg/mL ETD-249.09 -747.29μg/mL |
THC-3.05min ETD-2.10min. |
Degradation not in detail | [75] |
| 5 | THC+ACE | Tablet | Potassium phosphate monohydrate buffer (pH-5.0): Acetonitrile: Methanol in (40:20:40 % v/v) |
263 | THC -7.5 to 25μg/mL ACE-100-300µg/mL |
THC-2.8 min ACE-4.2 min |
Not completely explored | [76] |
| 6 | THC+DCF | Tablet | Solvent A (5 mM sodium dihydrogen phosphate, pH 2.5) and Solvent B (Methanol) |
258 | THC-40.48–121.43 µg/mL DCF 24.91–74 µg/mL |
THC-5.8 min. DCF-11.0 min. |
Good resolution | [77] |
| 7 | THC+DCF | Tablet | Methanol: Acetonitrile : Phosphate buffer (40:20:40 v/v at pH 5.0) | 263 | THC-7.5-25 µg/mL DCF-100-300µg/mL |
THC-2.8 min ACE-4.2 min | Separation not suitable in case of impurities | [78] |
| 8 | THC+ACE | Tablet | Methanol: Acetonitrile: THF: Acetate buffer (56:4:10:30 v/v) pH adjusted to 6.5 with Acetic acid | 312 | THC-0.4- 24 µg/mL ACE-10 -600 µg/ML |
THC-4.7 min. ACE-6.3min. |
Complex mobile phase | [79] |
| 9 | THC+ACE | Tablet | (A) 10 mM Ammonium acetate pH 5.00 buffer and (B) Acetonitrile: Water (70:30 v/v) | 265 | ACF-80-280 µg/mL THC- 6.4–22.4 µg/mL |
THC-13.29min ACF-2.20 min | Higher Rt | [80] |
| 10 | THC+ACE | Tablet | 5% ammonium acetate buffer and methanol (40:60 v/v) pH 5 with OPA | 276 | THC-4.8 -7.2 µg/mL ACE-63.8 -96 µg/mL |
THC-0.697 min. ACE-1.125 min. | Very low Rt | [81] |
| 11 | THC+ KET |
Tablet | Methanol: Toluene: Benzene (2.5:3.5:4 v/v) |
260 | 125-750 ng/band THC 1000-6000 ng/band KET |
THC 0.35 and KET 0.65 min. |
Well resolved components | [82] |
| 12 | THC+PCM+ DCF | Capsule | Acetonitrile: Phosphate buffer adjusted pH 3 with OPA | 228 | 100- 500 μg/mL PCM, THC and DCF | PCM -5.3;THC-9.61;DCF- 21.47 | Economical but not suitable as stability indicating | [83] |