| Factors | Levels | |
|---|---|---|
| -1 | +1 | |
| (A) Millimole(mM) of surfactant and cholesterol | 10 | 30 |
| (B) Surfactant:cholesterol ratio | 1:1 | 7:1 |
| (C) Time of hydration (hr) | 0.5 | 1 |
| (D) pH of hydration medium | 6.8 | 7.4 |
| (E) Hydration temperature (°C) | 55 | 65 |
| (F) Speed of rotation (rpm) | 60 | 80 |