| ➢ Simple and effective method for tolDC generation, compliant with current GMP regulations |
| ➢ Expression of MHC class II and the ability to process and present antigens |
| ➢ Low or intermediate expression of co-stimulatory molecules (e.g. CD80, CD86) |
| ➢ Anti-inflammatory cytokine profile: Low production of IL-12p70, IL-23, high production of IL-10 |
| ➢ Stable in a pro-inflammatory environment |
| ➢ Ability to induce T cell tolerance (e.g. through anergy, deletion, Tregs or immune deviation) in both nave and memory T cells |
| ➢ Ability to migrate to secondary lymphoid tissue through appropriate chemokine receptors (e.g. CCR7) |