Diagram of laboratory assay of Hypovirulent Binucleate Rhizoctonia (HBNR) to suppress the disease development of Fusarium Crown and Root Rot (FCRR) of tomato and to induce resistance against the disease, using the water agar method. (A) Inoculation point of HBNR consisting of a living mycelial disk (3-mm diameter), a dead mycelial disk (7-mm diameter), and CF (70 µl). In order to avoid direct contact between HBNR and FORL, the mycelial disk of living cells was enveloped by a polycarbonate membrane filter (0.2-µm mesh); (B) Inoculation point of Fusarium oxysporum f.sp. Radicis-Lycopersici (FORL) with spore suspension (5 µl of pathogen suspension at 5 × 105 spores/ml) at 0, 3, 6, and 9 cm away from the position of HBNR inoculum (separate experiment for each position); (C) Tomato root.
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