Table 1: Effect of Hypovirulent Binucleate Rhizoctonia (HBNR) with various pre-incubation times on the reduction of lesion development of Fusarium Crown and Root Rot (FCRR) of tomato caused by Fusarium Oxysporum f.sp. Radicis Lycopersici (FORL) in water agar a.
Treatments |
Lesion Development (cm)b
|
3 cmc
|
6 cm |
-12d
|
0 |
12 |
24 |
-12 |
0 |
12 |
24 |
Pathogen |
7.2 be
|
7.0 b |
6.7 b |
7.0 b |
6.4 b |
6.1 b |
5.6 b |
6.1 b |
HBNR W1 |
0.8 a |
0.8 a |
0.7 a |
0.5 a |
2.2 a |
1.8 a |
1.6 a |
1.1 a |
HBNR Rhv7 |
1.4 a |
0.7 a |
0.6 a |
0.7 a |
2.6 a |
2.2 a |
1.6 a |
1.6 a |
a Eight-day-old tomato seedlings were grown in 2% water agar treated with HBNR and challenge-inoculated with FORL.
b Lesion development was recorded 8 days after inoculation with FORL.
c Inoculation points of FORL were 3 cm and 6 cm away from HBNR position.
d Pre-incubation of HBNR on neck root: 12 h after inoculation of pathogen (-12); simultaneous inoculation of HBNR and pathogen (0 h); 12 h before inoculation of pathogen (12); 24 h before inoculation of pathogen (24).
e Mean of four replications with five seedlings per replication. Values followed by the same letter do not differ significantly (P > 0.05) according to Fisher’s least significant difference test.