| Target Gene | Primers Name | Sequence (5'-3') | PCR Program |
|---|---|---|---|
| arcC |
arcC1 arcC4 |
AGC CAT CTA CCG ACT AAC AC TCT GAA AGG GTT TGG CTA GC |
34×(94°C 30 s, 52°C 30 s, 72°C 1 min) |
| nrdE |
nrdE1 nrdE4 |
TTC TCC TTC AGG TGA CAG ATG AGA CTA GGC GTT TGA ACC TG |
34×(94°C 30 s, 54°C 30 s, 72°C 1 min) |
| proS |
proS1 proS4 |
TTG GTT GGA AAT GAC CAG ATC CCT GAT CCT TGA CAT TAA CGG |
34×(94°C 30 s, 51°C 30 s, 72°C 1 min) |
| spi |
spi1 spi4 |
CTT AGA GCA TTG CGC TAA GC TTG CCT GCT ATC TAG GGA AG |
34×(94°C 30 s, 50°C 30 s, 72°C 1 min) |
| tdk |
tdk1 tdk4 |
GAA TTC AGG AAA GAC CAT TG TAA TGC TTG CGA CAA ACT GG |
34×(94°C 30 s, 54°C 30 s, 72°C 1 min) |
| tpi |
tpi1 tpi4 |
GGC AGT AGT AAG CAA ATT ACC AGC AAG GCA AGG AAG CTA TC |
34×(94°C 30 s, 51°C 30 s, 72°C 1 min) |
| yqiL |
yqiL1 yqiL4 |
CCA CAT GGG AAT TAC AGC AG TCA ATA CAG ACG TCC CTT GAC C |
34×(94°C 30 s, 53°C 30 s, 72°C 1 min) |