Table 3: Biochemical characterisation of bacteria from concrete and earthen fish ponds in Ile-Ife, Osun State.

Isolate
Code
Cultural Characteristics on Agar Cell Shape Gram Reaction Catalase Citrate Indole Coagulase Suspected
Organism
C101 Pink, round, entire flat on EMB agar R - N.A + - - Klebsiella sp.
C102 Greenish metallic sheen, irregular, entire, flat on EMB agar R - N.A - + - E. coli
C201 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
E101 Pink, round, entire, convex on EMB agar R - N.A + - - Klebsiella sp.
E102 Pink, round, entire, flat on EMB agar R - N.A + - - Klebsiella sp.
E201 Yellow, round, entire, flat on NAG C + + + - + S. aureus
E202 Cream, irregular, entire, raised on NAG C - N.A + - - Enterococcus sp.
E203 Blue. round, entire, flat on EMB agar R - N.A + - - Enterobacter sp.
E204 Purple, round, entire, flat on EMB agar R - N.A + - - Enterobacter sp.
E205 Pink, round, entire flat on EMB agar R - N.A + - - Klebsiella sp.
E206 Pink, round, entire, raised on EMB agar R - N.A + - - Klebsiella sp.
E207 Pink, round, entire, flat SSA R - N.A - + - E. coli
E208 Pink, round, entire flat on EMB agar R - N.A + - - Klebsiella sp.
E209 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
E210 Purple, round, entire, raised on EMB agar R - N.A + - - Enterobacter sp.

C: Cocci, R: Rod, -: Negative, +: positive, S. aureus: Staphylococcus aureus, sp.: species, NAG: Nutrient agar, EMB: Eosine methylene blue, SSA: Salmonella and Shigella agar; N.A: Not applicable