Table 4: Bacteria isolation and biochemical characterization of bacteria from tank ponds in Ile-Ife, Osun State.

Isolate
Code		 Cultural Characteristics on Agar Cell Shape Gram Reaction Catalase Citrate Indole Coagulase Suspected Organism
T101 Cream, round entire flat on EMB agar R - N.A + - - Klebsiella sp.
T102 Cream round entire raised on NAG C + + + - - Staphylococcus sp.
T103 Pink, irregular, entire, flat on SSA R - N.A - + - E. coli
T104 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
T105 Pink, irregular, entire, flat on SSA R - N.A - + - E. coli
T106 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
T107 Greenish metallic sheen, irregular, entire, flat on EMB agar R - N.A - + - E. coli
T108 Blue, round, entire, flat on EMB agar R - N.A + - - Enterobacter sp.
T109 Purple, irregular, entire, flat on EMB agar R - N.A + - - Enterobacter sp.
T110 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
T111 Pink, round, entire, raised on EMB agar R - N.A + - - Klebsiella sp.
T112 Purple, irregular, entire, flat on EMB agar R - N.A + - - Enterobacter sp.
T113 Greenish metallic sheen, irregular, entire, flat on EMB agar R - N.A - + - E. coli
T114 Pink, round, entire, raised on EMB agar R - N.A + - - Klebsiella sp.
T201 Yellow, round, entire, flat on NA C + +ve + - + S. aureus
T202 Yellow, round, entire, flat on NA C + +ve + - + S. aureus
T203 Pink, irregular, entire, flat on SSA R - N.A _ + - E. coli
T204 Black, round, entire, flat on SSA R - N.A + - - Salmonella sp.
T205 Pink, round, entire, raised on EMB agar R - N.A + - - Klebsiella sp.
T206 Blue, round, entire, flat on EMB agar R - N.A + - - Enterobacter sp.

C: Cocci, R: Rod, -: Negative, +: positive, S. aureus: Staphylococcus aureus, sp.: species, EMB: Eosine methylene blue, SSA: Salmonella and Shigella agar; N.A: Not applicable