Fig. (1) MTT reduction and LDH release of primary neuronal cells after 24 hours exposure to 1.5 Vol% isoflurane (black) or controls (white) in the presence or absence of the GABAA receptor antagonists bicuculline and picrotoxin. Comparison of immature cells 5 DIV (A) and (B), and mature cells 15 DIV (C) and D). Data are means ± SEM; n = 9; one-way ANOVA test with Bonferroni post hoc test; ** P<0.01; ***P<0.001.