| Inclusion Criteria | Exclusion Criteria |
|---|---|
| 1. The volunteers were healthy with ages above 18 years. | 1. The volunteer had oral lesions on the buccal mucosa. |
| 2. The volunteers had the same number of teeth on the left and right side of their mouth. | 2. The volunteer had poor blood circulation, |
| 3. The volunteers had ASA physical status of I or II. | 3. The volunteer had allergies to chlorhexidine or lidocaine. |
| 4. The volunteers had the intact oral mucosal membrane. | 4. The volunteer use antimicrobial mouthwash prior to the experiment. |
| 5. The volunteer received antibiotics within 2 weeks prior to the study. |