Fig. (5) SG-12 promoted the progression of late stage of Fasmediated cell death. (A, B) A20/2J cells (1 × 10⁶ cells/ml) were incubated without or with anti-mouse Fas mAb or 8 µM SG-12 for 20 h in the absence (A) or presence (B) of 5 µg/ml anti-mouse IgG pAb. Values are means ± SD from triplicate samples. Statistically significant differences (*p < 0.05 or **p < 0.01) with respect to the values without SG-12 are indicated. Results are representative of four independent experiments. (C, D) A20/2J cells (1 × 10⁶ cells/ml) were incubated without or with anti-mouse Fas mAb without (C) or with (D) 5 µg/ml F(ab’)₂ of anti-mouse IgG pAb in the presence or absence of 8 µM SG-12. After 20 h, the cells were lysed and their proteins were detected by immunoblotting as described in “Materials and Methods.” Essentially the same result was obtained in another independent experiment.