Fig. (6) Effect of SG-12 on Fas-mediated cell death was relieved by transfection of SPHK2 but not SPHK1. A20/2J cells (A, B) or the cells transfected with SPHK1 (C, D) or SPHK2 (E, F) were incubated with 100 ng/ml anti-mouse Fas mAb for 20 h without (A, C, E) or with (B, D, F) 5 µg/ml F(ab’)2 of anti-mouse IgG pAb in the presence of indicated concentrations of SG-12. Values are means ± SD from triplicate samples. Statistically significant differences (*p < 0.05 or **p < 0.01) with respect to the values without SG-12 are indicated. Essentially the same result was obtained in an independent experiment.