Fig. (4) Time-dependent inhibition of soybean lipoxygenase-1 in
the presence of tetradecyl gallate (10). (A) Conditions were as follows:
40 μM linoleic acid, 1.3 nM lipoxygenase-1, and concentrations
of tetradecyl gallate for curves 0-8 were 0, 0.04, 0.08, 0.1, 0.2,
0.4, 0.6, 1.0, and 4.0 µM. The kobs values at each inhibitor concentrations
were determined by fitting the data to eq 1. (B) Dependence
of the values for kobs on the concentration of tetradecyl gallate. The
kobs values, determined in panel A, were fitted to eq 2.
